Addgene - pFloxin-Ofd1-Myc-KDD359-361FSY Plasmid Data

Plasmid 24578: pFloxin-Ofd1-Myc-KDD359-361FSY

Gene/insert name:
Ofd1
Insert size:
2781
Species:
M. musculus (mouse)
GenBank ID:
NM_177429
Entrez Gene:
Ofd1 (ORF2, Cxorf5, DXGgc7e)
Fusion protein or tag:
Myc
Terminal:
C terminal on insert
Mutation:
Lysine-Aspartate-Aspartate 359-361 changed to Phenylalanine-Serine-Tyrosine (codons AAG-GAT-GAC changed to TTT-TTC-TAC) Includes exons 4-23 only
Vector backbone:
pBluescript
(Search Vector Database)
Vector type:
Mouse Targeting, Cre/Lox
Backbone size w/o insert (bp):
4021
5' sequencing primer:
aacctctgccctttctcctc List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl2
Growth temperature (℃):
37
Growth instructions:
Stbl2 (Invitrogen) or other RecA- strain 37 degrees in LB media
High or low copy:
Low Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Jeremy Reiter
Terms and Licenses:
MTA

Comments:

Mutation reported in:

Identification of the gene for oral-facial-digital type I syndrome.

Ferrante MI, Giorgio G, Feather SA, Bulfone A, Wright V, Ghiani M, Selicorni A, Gammaro L, Scolari F, Woolf AS, Sylvie O, Bernard L, Malcolm S, Winter R, Ballabio A, Franco B.

Am J Hum Genet. 2001 Mar;68(3):569-76. Epub 2001 Feb 13.PMID: 11179005

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Ofd1, a human disease gene, regulates the length and distal structure of centrioles. Singla et al (Dev Cell. 2010 Mar 16. 18(3):410-24. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24578" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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