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pRSFDUET-ssDsbA-mCherry2 VNp6-mNeongreen
(Plasmid #245929)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245929 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRSFDUET-1
  • Total vector size (bp) 5362
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    ssDsbA-mCherry2
  • Species
    Synthetic
  • Insert Size (bp)
    843
  • Promoter T7
  • Tag / Fusion Protein
    • mCherry2 (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer ACYCDuetUP1
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    VNp6-mNeongreen
  • Species
    Synthetic
  • Insert Size (bp)
    885
  • Promoter T7
  • Tag / Fusion Protein
    • mNeongreen (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer DuetUP2 Primer
  • 3′ sequencing primer T7 Terminator Primer
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Yes

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSFDUET-ssDsbA-mCherry2 VNp6-mNeongreen was a gift from Dan Mulvihill (Addgene plasmid # 245929 ; http://n2t.net/addgene:245929 ; RRID:Addgene_245929)

  • For your References section:

    Vesicle Nucleation Peptide Fusion Induced Extracellular Vesicles Are Distinct From Escherichia coli Outer Membrane Vesicles, and Provide an Enhanced Platform for Protein Production and Purification. Streather BR, Eastwood TA, Baker K, Liang M, Bailie AE, van der Velden TT, Jeuken LJC, Botchway SW, Wang L, Mulvihill DP. J Extracell Vesicles. 2026 Aug;15(8):e70354. doi: 10.1002/jev2.70354. 10.1002/jev2.70354 PubMed 42599979
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