Addgene - pFloxin-MCS2-IRES-MCS Plasmid Data

Plasmid 24642: pFloxin-MCS2-IRES-MCS

Gene/insert name:
None
Vector backbone:
pBluescript
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Mouse Targeting, Cre/Lox
Backbone size (bp):
4512
5' sequencing primer:
aacctctgccctttctcctc List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl2
Growth temperature (℃):
37
Growth instructions:
Stbl2 (Invitrogen) or any other RecA- strain 37 degrees in LB media
High or low copy:
Low Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Jeremy Reiter
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Floxin, a resource for genetically engineering mouse ESCs. Singla et al (Nat Methods. 2010 Jan . 7(1):50-2. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24642" in your Materials and Methods section.