pCS2 xenopus sizzled HA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24683||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesX. laevis (frog)
Insert Size (bp)800
Entrez Geneszl (a.k.a. xszl)
/ Fusion Protein
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
HA tag: YPYDVPVYVL
silent mutation at (bp) 657 of szl
E248D does not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2 xenopus sizzled HA was a gift from Edward De Robertis (Addgene plasmid # 24683 ; http://n2t.net/addgene:24683 ; RRID:Addgene_24683)
For your References section:Embryonic dorsal-ventral signaling: secreted frizzled-related proteins as inhibitors of tolloid proteinases. Lee HX, Ambrosio AL, Reversade B, De Robertis EM. Cell. 2006 Jan 13. 124(1):147-59. 10.1016/j.cell.2005.12.018 PubMed 16413488
Map uploaded by the depositor.