pMB.MBP.JARID2
(Plasmid
#247018)
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PurposeExpression of MBP-JARID2 in insect cells, MBP tags are C3-cleavable (PreScission Protease)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247018 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepMB.Pos6
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Gentamicin, 100 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameJARID2
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SpeciesH. sapiens (human)
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Entrez GeneJARID2 (a.k.a. DIDDF, JMJ)
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Tag
/ Fusion Protein
- MBP (N terminal on insert)
Cloning Information
- Cloning method Golden Gate
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMB.MBP.JARID2 was a gift from Chen Davidovich (Addgene plasmid # 247018 ; http://n2t.net/addgene:247018 ; RRID:Addgene_247018) -
For your References section:
Auto-inhibition of PRC2 by the broadly expressed long isoform of AEBP2. Mucha M, Lai Z, McKenzie NJ, Matra F, Boudes M, Flanigan SF, Alejo-Vinogradova MT, Monger C, Zhang Q, Nimmo D, Healy E, Silva AJ, Angelov D, Reck DM, Holland G, Atmaca ZE, King HE, Hamilton M, Glancy E, Nolan J, Weatheritt RJ, Bell O, Vermeulen M, Davidovich C, Bracken AP. EMBO J. 2025 Dec;44(23):6979-7020. doi: 10.1038/s44318-025-00616-9. Epub 2025 Oct 30. 10.1038/s44318-025-00616-9 PubMed 41168462