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Puro 2.0
(Plasmid #24970)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    Puro 2.0
  • Backbone size (bp) 7705
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer MSCV-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

This vector was derived from pSICO-pgk Puro, and no longer contains the loxp sites. Therefore, Puro 2.0 allows constitutive shRNA expression in mammalian cells. The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on the Jacks Lab website http://web.mit.edu/ccr/labs/jacks/protocols/pSico.html

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Puro 2.0 was a gift from Tyler Jacks (Addgene plasmid # 24970)
  • For your References section:

    Tissue-specific p19Arf regulation dictates the response to oncogenic K-ras. Young NP, Jacks T. Proc Natl Acad Sci U S A. 2010 Jun 1. 107(22):10184-9. 10.1073/pnas.1004796107 PubMed 20479239