|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24970||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonePuro 2.0
- Backbone size (bp) 7705
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
This vector was derived from pSICO-pgk Puro, and no longer contains the loxp sites. Therefore, Puro 2.0 allows constitutive shRNA expression in mammalian cells. The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on the Jacks Lab website http://web.mit.edu/ccr/labs/jacks/protocols/pSico.html
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Puro 2.0 was a gift from Tyler Jacks (Addgene plasmid # 24970)
For your References section:Tissue-specific p19Arf regulation dictates the response to oncogenic K-ras. Young NP, Jacks T. Proc Natl Acad Sci U S A. 2010 Jun 1. 107(22):10184-9. 10.1073/pnas.1004796107 PubMed 20479239
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.