1
Plasmid 24970: Puro 2.0
  • None

  • Puro 2.0
    (Search Vector Database)

  • Mammalian Expression, Lentiviral, RNAi

  • 7705

  • HpaI

  • No

  • XhoI

  • No

  • CMV-F List of Sequencing Primers

  • MSCV-rev

  • Ampicillin

  • Stbl3

  • 37

  • Stbl3

  • High Copy

  • Puromycin

  • View sequences (2)
  • View map

  • Tyler Jacks

  • UBMTA

Comments: 

This vector was derived from pSICO-pgk Puro, and no longer contains the loxp sites. Therefore, Puro 2.0 allows constitutive shRNA expression in mammalian cells. The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on the Jacks Lab website http://web.mit.edu/ccr/labs/jacks/protocols/pSi...

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Tissue-specific p19Arf regulation dictates the response to oncogenic K-ras. Young et al (Proc Natl Acad Sci U S A. 2010 Jun 1. 107(22):10184-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24970" in your Materials and Methods section.