|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24979||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameFrzb-1 HA
SpeciesX. laevis (frog)
Insert Size (bp)1400
/ Fusion Protein
- HA tag internal (YPYDVPDYA)
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
For sense, cut with NotI, transcribe with SP6.
HA tag is cloned between Ser158 and Met159 of Frezzled by PCR. HA tag includes a AatII diagnostic site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2 xFrzb-HA was a gift from Edward De Robertis (Addgene plasmid # 24979 ; http://n2t.net/addgene:24979 ; RRID:Addgene_24979)
For your References section:Frzb-1 is a secreted antagonist of Wnt signaling expressed in the Spemann organizer. Leyns L, Bouwmeester T, Kim SH, Piccolo S, De Robertis EM. Cell. 1997 Mar 21. 88(6):747-56. 10.1016/S0092-8674(00)81921-2 PubMed 9118218