Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25053||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7767
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)7584
MutationContains only the ROC domain- aa 1333-1516
Entrez GeneLRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
/ Fusion Protein
- GFP (N terminal on insert)
- Cloning method Gateway Cloning
- 5′ cloning site attR1 (not destroyed)
- 3′ cloning site attR2 (not destroyed)
- 5′ sequencing primer CMV-F, GFP-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The domain structure and amino acid limits is approximate. According to depositor's domain organization, this construct contains the correct residues.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDEST53-LRRK2-ROC was a gift from Mark Cookson (Addgene plasmid # 25053 ; http://n2t.net/addgene:25053 ; RRID:Addgene_25053)
For your References section:Kinase activity is required for the toxic effects of mutant LRRK2/dardarin. Greggio E, Jain S, Kingsbury A, Bandopadhyay R, Lewis P, Kaganovich A, van der Brug MP, Beilina A, Blackinton J, Thomas KJ, Ahmad R, Miller DW, Kesavapany S, Singleton A, Lees A, Harvey RJ, Harvey K, Cookson MR. Neurobiol Dis. 2006 Aug . 23(2):329-41. 10.1016/j.nbd.2006.04.001 PubMed 16750377