Addgene - pCDNA3 Flag BimL(S58D,DE) Plasmid Data

Plasmid 25061: pCDNA3 Flag BimL(S58D,DE)

Gene/insert name:
BimL (S58D,DE)
Alt name:
BCL2-like 11
Insert size:
458
Species:
H. sapiens (human)
GenBank ID:
AF032458
Entrez Gene:
BCL2L11 (BAM, BIM, BIM-alpha6, BIM-beta6, BIM-beta7, BOD, BimEL, BimL, BimS)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Mutation:
Changed serine 58 to aspartic acid changed leucine 92 to aspartic acid changed isoleucine 95 to glutamic acid
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5446
Cloning site 5':
Hind III
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Roger Davis
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: JNK phosphorylation of Bim-related members of the Bcl2 family induces Bax-dependent apoptosis. Lei et al (Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25061" in your Materials and Methods section.

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