Addgene - pGSTag BimL(AAA) Plasmid Data

Plasmid 25063: pGSTag BimL(AAA)

Gene/insert name:
BimL (AAA)
Alt name:
BCL2-like 11
Insert size:
257
Species:
H. sapiens (human)
Entrez Gene:
BCL2L11 (BAM, BIM, BIM-alpha6, BIM-beta6, BIM-beta7, BOD, BimEL, BimL, BimS)
Fusion protein or tag:
Gst
Terminal:
N terminal on backbone
Mutation:
Changed serine 44 to alanine Changed threonine 56 to alanine Changed serine 58 to alanine
Vector backbone:
pGSTag
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5057
Cloning site 5':
Bam HI
Site destroyed during cloning:
No
Cloning site 3':
Hind III
Site destroyed during cloning:
No
5' sequencing primer:
pGex 5' List of Sequencing Primers
3' sequencing primer:
pGex 3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Roger Davis
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: JNK phosphorylation of Bim-related members of the Bcl2 family induces Bax-dependent apoptosis. Lei et al (Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25063" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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