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pET28a-pro-sumo2
(Plasmid #25102)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25102 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha for plasmid purification. BL21(DE3) for protein production.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pro-SUMO2
  • Alt name
    Small ubiquitin-related modifier 2
  • Alt name
    Sentrin 2
  • Alt name
    Ubiquitin-like protein SMT3B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    312
  • GenBank ID
    AK311837
  • Entrez Gene
    SUMO2 (a.k.a. HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A)
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-pro-sumo2 was a gift from Guy Salvesen (Addgene plasmid # 25102 ; http://n2t.net/addgene:25102 ; RRID:Addgene_25102)
  • For your References section:

    Small ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs. Mikolajczyk J, Drag M, Bekes M, Cao JT, Ronai Z, Salvesen GS. J Biol Chem. 2007 Sep 7. 282(36):26217-24. 10.1074/jbc.M702444200 PubMed 17591783