pSPIN-GG
(Plasmid
#251750)
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Purpose(Empty Backbone) Single-plasmid V. cholerae CAST encoding proteins, crRNA, and donor DNA. Entry vector: GFP dropout; BbsI spacer, BsaI promoter swap, SAPI donor cloning; pSC101* backbone.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251750 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepSC101*
- Backbone size (bp) 13712
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Vector typeBacterial Expression, CRISPR, Synthetic Biology
- Promoter J23119
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Marionette DH10b
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Growth instructionsUse Marionette DH10b strain (sAJM.1504, Addgene #108251) as this avoids expression of the CRISPR machinery encoded and greatly enhances stability.
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Copy numberLow Copy
Cloning Information
- Cloning method Golden Gate
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSPIN-GG was a gift from Thomas Gorochowski (Addgene plasmid # 251750 ; http://n2t.net/addgene:251750 ; RRID:Addgene_251750) -
For your References section:
A Golden Gate-Compatible CRISPR-Associated Transposon Tool for Multiplexed Bacterial Genome Editing. Irvine TCT, Bailey AM, Gorochowski TE. Methods Mol Biol. 2026;3041:33-45. doi: 10.1007/978-1-0716-5304-3_2. 10.1007/978-1-0716-5304-3_2 PubMed 42420722
Map uploaded by the depositor.
