|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25365||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepCMV-Tag 3B
Backbone manufacturerCookson Lab
- Backbone size w/o insert (bp) 4300
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)7581
MutationLysine 1347 mutated to Alanine
Entrez GeneLRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
/ Fusion Protein
- 2XMyc (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
An additional Myc tag has been added to the original vector. The 2XMyc vector was adapted to the invitrogen gateway system by insertion of a gateway cloning cassette. LRRK2 was cloned by gateway recombination.
In order to provide these LRRK2 plasmids as a resource to the community, we depend on the contributing academic laboratory for quality control. Therefore, the full-length LRRK2 plasmids are distributed in the format provided by the contributing institution "as is" with no additional guarantee.
Addgene is not responsible for any errors in these constructs.
It is strongly encouraged that a diagnostic digest be performed on this plasmid before use in an experiment (see attached PDF).
If there is an issue with your stock, please contact the Cookson lab for a replacement.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:2XMyc-LRRK2-K1347A was a gift from Mark Cookson (Addgene plasmid # 25365 ; http://n2t.net/addgene:25365 ; RRID:Addgene_25365)
For your References section:The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation. . J Biol Chem. 2008 Jun 13;283(24):16906-14. 10.1074/jbc.M708718200 PubMed 18397888