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pLEX307-puro-SARS-CoV-2-nsp4-nsp5-L50F-E166A-P168S-nsp6
(Plasmid #254475)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254475 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLEX307
  • Backbone manufacturer
    David Root (Addgene #41392)
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 3CL protease with L50F, E166A, and P168S fused to nsp4 and nsp6
  • Species
    SARS-CoV-2
  • Mutation
    L50F, E166A, and P168S

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We have noticed that bacterial transposons commonly arise in this series of plasmids, and suggest checking the integrity of the plasmids prior to use.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX307-puro-SARS-CoV-2-nsp4-nsp5-L50F-E166A-P168S-nsp6 was a gift from Sho Iketani (Addgene plasmid # 254475 ; http://n2t.net/addgene:254475 ; RRID:Addgene_254475)

  • For your References section:

    SARS-CoV-2 resistance pathways to EDP-235. Wang K, Gibbons JS, Bisht N, Reyes AC, Cressey TN, Crepeau ML, Balakrishnan A, Kelly NM, Bai T, Lovett DH, Torres Yanagisawa AE, Caccin FNM, Rhodin MHJ, Iketani S. Antiviral Res. 2026 Sep;253:106493. doi: 10.1016/j.antiviral.2026.106493. Epub 2026 Jul 27. 10.1016/j.antiviral.2026.106493 PubMed 42508618
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