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MBP-His6-TEV-MMS2
(Plasmid #25465)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pMalC2XHV
  • Backbone manufacturer
    modified from NEB vector
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21(DE3) RIL (chloramphenicol) 37C growth to O.D.=0.6 induce with 1mM IPTG @16C overnight
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    MMS2
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    414
  • GenBank ID
    NP_011428
  • Entrez Gene
    MMS2 (a.k.a. YGL087C)
  • Entrez Gene
    UBC13 (a.k.a. YDR092W)
  • Tags / Fusion Proteins
    • MBP (N terminal on insert)
    • His6 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer MalE
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MBP-His6-TEV-MMS2 was a gift from Cynthia Wolberger (Addgene plasmid # 25465)
  • For your References section:

    Mms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation. Eddins MJ, Carlile CM, Gomez KM, Pickart CM, Wolberger C. Nat Struct Mol Biol. 2006 Oct . 13(10):915-20. 10.1038/nsmb1148 PubMed 16980971