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Plasmid 25465: MBP-His6-TEV-MMS2
  • MMS2

  • 414

  • S. cerevisiae (budding yeast)

  • NP_011428

  • MMS2 (YGL087C)
    UBC13 (YDR092W)

  • MBP

  • N terminal on insert

  • His6

  • N terminal on insert

  • pMalC2XHV
    (Search Vector Database)

  • modified from NEB vector

  • Bacterial Expression

  • BamHI

  • No

  • HindIII

  • No

  • MalE List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • BL21(DE3) RIL (chloramphenicol) 37C growth to O.D.=0.6 induce with 1mM IPTG @16C overnight

  • High Copy

  • View sequences (1)
  • Cynthia Wolberger

  • UBMTA

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Mms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation. Eddins et al (Nat Struct Mol Biol. 2006 Oct . 13(10):915-20. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25465" in your Materials and Methods section.