AAV-S5E2-jGCaMP8f-WPRE
(Plasmid #255452)

• Purpose: AAV vector to drive the expression of GCaMP8f cortical interneurons under the control of the E2 regulatory element.
• Depositing Lab: Eric Melonakos
• Publication: AAV-S5E2-JGCaMP8f-WPRE (unpublished)

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Backbone

• Vector backbone: pAAV-S5E2-GCaMP6f
• Backbone manufacturer: Addgene #135632
• Backbone size w/o insert (bp): 4698
• Total vector size (bp): 6051
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: jGCaMP8f
• Species: R. norvegicus (rat), G. gallus (chicken); A. victoria (jellyfish)
• Insert Size (bp): 1355
• Promoter: S5E2
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (1125) (destroyed during cloning)
• 3′ cloning site: HindIII (2473); [NcoI (1090)-NheI (1125) blunt ligation] (unknown if destroyed)
• 5′ sequencing primer: pBR322ori-F

Gene/Insert 2

• Gene/Insert name: jGCaMP8f

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry