Addgene - IRS1-A4 Plasmid Data

Plasmid 25697: IRS1-A4

Gene/insert name:
IRS1
Species:
H. sapiens (human)
Entrez Gene:
IRS1 (HIRS-1)
Vector backbone:
pSUPERIOR RETRO-PURO
(Search Vector Database)
Backbone manufacturer:
Oligoengine
Vector type:
Retroviral, RNAi
Backbone size w/o insert (bp):
6349
5' sequencing primer:
pLXSN5' List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Eric Fearon
Terms and Licenses:
MTA

Comments:

sense 5′-GAT CCC GAG CAT GTA CAA ATG CTT CTC TTC CTG TCA AGA AGC GTT TGT GCA TGC TCT TTT TA-3′

antisense 5′-AGC TTA AAA AGA GCA TGC ACA AAC GCT TCT TGA CAG GAA GAG AAG CAT TTG TAC ATG CTC GG

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: IRS1 regulation by Wnt/beta-catenin signaling and varied contribution of IRS1 to the neoplastic phenotype. Bommer et al (J Biol Chem. 2010 Jan 15. 285(3):1928-38. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25697" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

14582: pSUPER.ATRi

32200: pcDNA3-tRNA scaffolded SA (tRSA)