Addgene - FUW-TetO-mLIN28 Plasmid Data

Plasmid 25699: FUW-TetO-mLIN28

Gene/insert name:
Lin28
Insert size:
642
Species:
M. musculus (mouse)
Entrez Gene:
Lin28a (RP23-314D24.1, AL024421, ENSMUSG00000070700, Gm10299, Lin-28, Lin28, Tex17, lin-28A)
Vector backbone:
FUW-TetO
(Search Vector Database)
Vector type:
Lentiviral
Backbone size w/o insert (bp):
8500
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
AAAGTGAAAGTCGAGCTCGGTACC List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Rudolf Jaenisch
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Direct cell reprogramming is a stochastic process amenable to acceleration. Hanna et al (Nature. 2009 Dec 3. 462(7273):595-601. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25699" in your Materials and Methods section.