200 bp Axin2 mutant
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25704||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameAxin 2 promoter
SpeciesH. sapiens (human)
Insert Size (bp)1000
MutationTCF DNA binding site at bp -108 to -102 mutated CTTTGAT --> CTTTGGC
Entrez GeneAXIN2 (a.k.a. AXIL, ODCRCS)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site NheI (unknown if destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Contains AXIN2 sequences from -181 to +5 relative to the presumed transcription start site.
Mutation listed above.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:200 bp Axin2 mutant was a gift from Eric Fearon (Addgene plasmid # 25704 ; http://n2t.net/addgene:25704 ; RRID:Addgene_25704)
For your References section:Activation of AXIN2 expression by beta-catenin-T cell factor. A feedback repressor pathway regulating Wnt signaling. Leung JY, Kolligs FT, Wu R, Zhai Y, Kuick R, Hanash S, Cho KR, Fearon ER. J Biol Chem. 2002 Jun 14. 277(24):21657-65. 10.1074/jbc.M200139200 PubMed 11940574