Sc RFC[2 + 3 + 4(GAT)]-pET
(Plasmid #258190)

• Purpose: Over-express in E. coli Sc RFC2, RFC3, & RFC4, with RFC4 containing a P-loop (GAT) mutation. Co-express with Sc RFC5 and Sc RFC1, rad24, ctf18, or Elg1.
• Depositing Lab: Michael O'Donnell
• Publication: O'Donnell Lab Plasmids May 2026 (unpublished)

Backbone

• Vector backbone: pET(11a) / pET(16b)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Sc RFC2, Sc RFC3, Sc RFC4; see relevant mutations
• Species: S. cerevisiae (budding yeast)
• Mutation: RFC4 (K55A) (GAT)
• Entrez Gene: RFC2 (a.k.a. YJR068W)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mix & Match (co-transform) with other vectors to over-express Sc RFC complex or related complexes (with Sc RFC1 substituted by Sc rad24, Sc ctf18, or Sc Elg1) containing the desired mutation(s). Expression in CodonPlus(RIL) or other strain containing rare Sc tRNA genes recommended.