cDNA or shRNA Library 25864: Monosiga cDNA library

• Gene/insert name: Monosiga brevicollis cDNA
• Species: Monosiga brevicollis
• Vector backbone: pSport 1
  (Search Vector Database)
• Backbone manufacturer: Invitrogen
• Vector type: Bacterial Expression
• Backbone size w/o insert (bp): 4110
• 5' sequencing primer: T7
• Bacterial resistance(s) Ampicillin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: High Copy
• Supplemental document: Notes from Addgene (1)
• Principal Investigator: Sean Carroll
• Principal Investigator: Nicole King
• Terms and Licenses: MTA

Comments: 

cDNA Library Construction. Double-stranded cDNA was synthesized with SuperScriptII RT and DNA polymerase I and ligated to adapters containing a SalI restriction site (GIBCO/BRL). To remove residual adapters and enrich for full-length fragments, cDNA was first size-fractionated by column chromatography and then ligated to predigested pSportI vector (GIBCO/BRL). Transformation of DH5α with the ligated DNA yielded a plasmid library with approximately 8.8 ´ 104 colony-forming units.

Note: Addgene has sequenced part of this library. See the Reviews listed on the right-hand column above for more information.

In order to provide this library to the scientific community at a reasonable cost, we are shipping this library as DNA in 100 ng/ul (100 ul total) aliquots at room temperature.

Article: A receptor tyrosine kinase from choanoflagellates: molecular insights into early animal evolution. King et al (Proc Natl Acad Sci U S A. 2001 Dec 18. 98(26):15032-7. PubMed)