1
Plasmid 25890: pLX304
  • None

  • V5

  • C terminal on backbone

  • pLKO
    (Search Vector Database)

  • Mammalian Expression, Lentiviral ; Gateway Destination vector

  • 9377

  • Gateway Cloning

  • CMV-F List of Sequencing Primers

  • WPRE-R

  • Ampicillin and Chloramphenicol

  • ccdB Survival

  • 30

  • ccdB resistant bacteria. 30C for liquid culture. 37C for plates.

  • High Copy

  • Blasticidin

  • View sequences (7)
  • pLX Gateway manual (application/pdf)

  • Notes from Addgene (1)

  • David Root

  • UBMTA

Comments: 

There is a C-terminal V5 tag followed by a Stop codon directly after the Gateway sequence.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A public genome-scale lentiviral expression library of human ORFs. Yang et al (Nat Methods. 2011 Jun 26. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25890" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only