Addgene - pLX302 Plasmid Data

Plasmid 25896: pLX302

Gene/insert name:
None
Fusion protein or tag:
V5
Terminal:
C terminal on backbone
Vector backbone:
pLKO
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral ; Gateway Destination vector
Backbone size (bp):
9573
Cloning method:
Gateway Cloning
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
WPRE-R
Bacterial resistance(s)
Ampicillin and Chloramphenicol
Growth strain(s)
ccdB Survival
Growth temperature (℃):
30
Growth instructions:
ccdB resistant bacteria. 30C for liquid culture (slow grower--allow 24 hours for growth). 37C for plates.
High or low copy:
Low Copy
Selectable markers:
Puromycin
Sequence: View sequences (6)
Supplemental document:
pLX Gateway manual (application/pdf)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
David Root
Terms and Licenses:
MTA

Comments:

There is a C-terminal V5 tag followed by a Stop codon directly after the Gateway sequence.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A public genome-scale lentiviral expression library of human ORFs. Yang et al (Nat Methods. 2011 Jun 26. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25896" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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