pLX302
(Plasmid #25896)

• Purpose: (Empty Backbone) 3rd generation lentiviral Gateway destination vector. Puromycin selection, V5 tag.
• Depositing Lab: David Root
• Depositing Organization: Broad Institute
  Located in United States of America
• Publication: Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
• Citations: 55 Articles

Backbone

• Vector backbone: pLKO
• Backbone size (bp): 9573
• Vector type: Mammalian Expression, Lentiviral, Gateway Cloning
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Growth instructions: ccdB resistant bacteria. 30C for liquid culture (slow grower--allow 24 hours for growth). 37C for plates.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a C-terminal V5 tag followed by a Stop codon directly after the Gateway sequence.