- Backbone size (bp) 9573
Vector typeMammalian Expression, Lentiviral ; Gateway Destination vector
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Chloramphenicol
Growth Strain(s)ccdB Survival
Growth instructionsccdB resistant bacteria. 30C for liquid culture (slow grower--allow 24 hours for growth). 37C for plates.
Copy numberLow Copy
/ Fusion Protein
- V5 (C terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
There is a C-terminal V5 tag followed by a Stop codon directly after the Gateway sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLX302 was a gift from David Root (Addgene plasmid # 25896)
For your References section:A public genome-scale lentiviral expression library of human ORFs. Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE. Nat Methods. 2011 Jun 26. ():. 10.1038/nmeth.1638 PubMed 21706014
Map generated by Addgene from full sequence supplied by depositor.