Fusion protein or tag:
C terminal on backbone
( Search Vector Database)
Mammalian Expression, Lentiviral ; Gateway Destination vector
Backbone size (bp):
5' sequencing primer:
List of Sequencing Primers
3' sequencing primer:
Ampicillin and Chloramphenicol
Growth temperature (℃):
ccdB resistant bacteria. 30C for liquid culture (slow grower--allow 24 hours for growth). 37C for plates.
High or low copy:
View sequences (6)
pLX Gateway manual (application/pdf)
Notes from Addgene (1)
Terms and Licenses:
sequenced a portion of this plasmid for verification.
Full plasmid sequence is available only if provided by the depositing laboratory.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
25896" in your Materials and Methods section.