pLX302
(Plasmid #25896)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pLKO
  • Backbone size (bp) 9573
  • Vector type
    Mammalian Expression, Lentiviral ; Gateway Destination vector
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Chloramphenicol
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdB resistant bacteria. 30C for liquid culture (slow grower--allow 24 hours for growth). 37C for plates.
  • Copy number
    Low Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

There is a C-terminal V5 tag followed by a Stop codon directly after the Gateway sequence.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX302 was a gift from David Root (Addgene plasmid # 25896)
  • For your References section:

    A public genome-scale lentiviral expression library of human ORFs. Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE. Nat Methods. 2011 Jun 26. ():. 10.1038/nmeth.1638 PubMed 21706014