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cp204
(Plasmid #25951)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25951 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cp204
  • Alt name
    cp204
  • Species
    D. Striata
  • Insert Size (bp)
    800
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer RECA1
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Addgene's sequence with pBAD-F shows minor mismatches and gaps with the reference sequence from the depositing lab. These discrepancies are a result of mistakes made during sequence assembly and should not impact plasmid function.

Please note that growing the construct at 30-34C may increase protein yield (36-48hours).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cp204 was a gift from Robert Campbell (Addgene plasmid # 25951)
  • For your References section:

    Circularly permuted monomeric red fluorescent proteins with new termini in the beta-sheet. Carlson HJ, Cotton DW, Campbell RE. Protein Sci. 2010 Jun 2. ():. 10.1002/pro.428 PubMed 20521333