Addgene - pOPINVH Plasmid Data

Plasmid 26041: pOPINVH

Gene/insert name:
u-LacZ-KHHHHHH
Fusion protein or tag:
u-phosphatase secretion leader
Terminal:
N terminal on insert
Fusion protein or tag:
KHHHHHH
Terminal:
C terminal on insert
Vector backbone:
pTriEx-2
(Search Vector Database)
Backbone manufacturer:
Novagen
Vector type:
Mammalian Expression, Bacterial Expression, Insect Expression
Backbone size w/o insert (bp):
5457
Cloning site 5':
NcoI
Site destroyed during cloning:
Unknown
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T7-term
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
pTriEx obtained from Merck
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Ray Owens
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A pipeline for the production of antibody fragments for structural studies using transient expression in HEK 293T cells. Nettleship et al (Protein Expr Purif. 2008 Nov . 62(1):83-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26041" in your Materials and Methods section.