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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26066 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCI-neo
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5472
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePitaire
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Alt nameCrkRS
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SpeciesH. sapiens (human)
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Entrez GeneCDK12 (a.k.a. CRK7, CRKR, CRKRS)
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Tags
/ Fusion Proteins
- His (N terminal on insert)
- Myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-Neo-His-Pitaire-Myc was a gift from Jonathon Pines (Addgene plasmid # 26066 ; http://n2t.net/addgene:26066 ; RRID:Addgene_26066) -
For your References section:
CrkRS: a novel conserved Cdc2-related protein kinase that colocalises with SC35 speckles. Ko TK, Kelly E, Pines J. J Cell Sci. 2001 Jul . 114(Pt 14):2591-603. PubMed 11683387