Addgene - pSVE Grb2 myc 49L/203R Plasmid Data

Plasmid 26087: pSVE Grb2 myc 49L/203R

Gene/insert name:
Grb2 49L/203R
Alt name:
Grb2
Insert size:
650
Species:
H. sapiens (human)
Entrez Gene:
GRB2 (ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2)
Fusion protein or tag:
myc
Terminal:
C terminal on insert
Mutation:
49L/203R
Vector backbone:
pSVE
(Search Vector Database)
Vector type:
Mammalian Expression
5' sequencing primer:
pBABE-3 List of Sequencing Primers
3' sequencing primer:
M13 reverse?
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

mutagenized with oligos from pSVE Grb2 myc 49L and pSVE Grb2 myc 203R together. Plasmid also known as pSVEGrb2myc u5j .

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Association of Sos Ras exchange protein with Grb2 is implicated in tyrosine kinase signal transduction and transformation. Egan et al (Nature. 1993 May 6. 363(6424):45-51. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26087" in your Materials and Methods section.