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pSVE Grb2 myc 49L/203R
(Plasmid #26087)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Grb2 49L/203R
  • Alt name
    Grb2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    650
  • Mutation
    49L/203R
  • Entrez Gene
    GRB2 (a.k.a. ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2)
  • Tag / Fusion Protein
    • myc (C terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

mutagenized with oligos from pSVE Grb2 myc 49L and pSVE Grb2 myc 203R together. Plasmid also known as pSVEGrb2myc u5j .

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSVE Grb2 myc 49L/203R was a gift from Bob Weinberg (Addgene plasmid # 26087)
  • For your References section:

    Association of Sos Ras exchange protein with Grb2 is implicated in tyrosine kinase signal transduction and transformation. Egan SE, Giddings BW, Brooks MW, Buday L, Sizeland AM, Weinberg RA. Nature. 1993 May 6. 363(6424):45-51. 10.1038/363045a0 PubMed 8479536