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pCM67
(Plasmid #261137)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 261137 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLF3FShC
  • Backbone manufacturer
    Michael Nonet, Addgene Plasmid #153083
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    elt-2p::AID::nls::mcherry::2a::AID::deltaPB1 PAR-6::VC155::tbb-2 3' in pLF3FShC
  • Species
    C. elegans (nematode)
  • Mutation
    deltaPB1 PAR-6
  • Entrez Gene
    par-6 (a.k.a. CELE_T26E3.3)
  • Promoter elt-2 minimal promoter

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCM67 was a gift from John Calarco (Addgene plasmid # 261137 ; http://n2t.net/addgene:261137 ; RRID:Addgene_261137)

  • For your References section:

    Spatio-temporal protein interaction analysis using bimolecular fluorescence complementation in C. elegans. Martin CJ, Calarco JA. bioRxiv 2025.02.28.640866 10.1101/2025.02.28.640866
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