pBS-PABPC
(Plasmid
#26129)
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Depositing Lab
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Publication
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Sequence Information
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Depositor Sequences: Partial (1)
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Addgene Sequences: Partial (1)
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 26129 | Plasmid sent as bacteria in agar stab | 1 | $65 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBluescript II SK(+)
- Backbone size w/o insert (bp) 3000
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Vector typecloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSmed-PABPC
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Alt namePoly(A)-binding protein, cytoplasmic
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SpeciesSchmidtea mediterranea
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Insert Size (bp)1787
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GenBank IDHM100651
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-PABPC was a gift from Phillip Newmark (Addgene plasmid # 26129) -
For your References section:
A functional genomic screen in planarians identifies novel regulators of germ cell development. Wang Y, Stary JM, Wilhelm JE, Newmark PA. Genes Dev. 2010 Sep 15. 24(18):2081-92. 10.1101/gad.1951010 PubMed 20844018