Addgene - pBS-PABPC Plasmid Data

Plasmid 26129: pBS-PABPC

Gene/insert name:
Smed-PABPC
Alt name:
Poly(A)-binding protein, cytoplasmic
Insert size:
1787
Species:
Schmidtea mediterranea
GenBank ID:
HM100651
Vector backbone:
pBluescript II SK(+)
(Search Vector Database)
Vector type:
cloning
Backbone size w/o insert (bp):
3000
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Phillip Newmark
Terms and Licenses:
UBMTA

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: A functional genomic screen in planarians identifies novel regulators of germ cell development. Wang et al (Genes Dev. 2010 Sep 15. 24(18):2081-92. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26129" in your Materials and Methods section.