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pBCN22-R4R3
(Plasmid #26302)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCG150
  • Backbone size (bp) 7401
  • Vector type
    Worm Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    Use DB3.1 E. coli (must be ccdB resistant). Note that ccdB Survival bacteria (Invitrogen) do not work for this plasmid.
  • Copy number
    Low Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AttR4 (destroyed during cloning)
  • 3′ cloning site AttR3 (destroyed during cloning)
  • 5′ sequencing primer M13 Reverse
  • 3′ sequencing primer M13 (-21) Forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Puromycin resistance gene from pBabePuro (Addgene). rpl-28 promoter and let-858 3'UTR sequences from pPD129.57 vector (Addgene). mCherry gene from pCG144 (Seydoux lab, Addgene). Pharyngeal enhancer (C183) with minimal myo-2 promoter amplified from genomic DNA of AZ218 worms (Caenorhabditis Genetics Center).
  • Terms and Licenses

Depositor Comments

Use for puromycin selection of transgenes in C. elegans.
This vector is identical to pBCN21-R4R3, but a 8xC183_minPmyo-2::mCherry::unc-54_3'UTR cassette was inserted in the backbone as an additional visual marker.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBCN22-R4R3 was a gift from Ben Lehner (Addgene plasmid # 26302)
  • For your References section:

    Rapid selection of transgenic C. elegans using antibiotic resistance. Semple JI, Garcia-Verdugo R, Lehner B. Nat Methods. 2010 Aug 22. ():. 10.1038/nmeth.1495 PubMed 20729840