Use DB3.1 E. coli (must be ccdB resistant). Note that ccdB Survival bacteria (Invitrogen) do not work for this plasmid.
Puromycin resistance gene from pBabePuro (Addgene).
rpl-28 promoter and let-858 3'UTR sequences from pPD129.57 vector (Addgene).
mCherry gene from pCG144 (Seydoux lab, Addgene).
Pharyngeal enhancer (C183) with minimal myo-2 promoter amplified from genomic DNA of AZ218 worms (Caenorhabditis Genetics Center).
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
26302" in your Materials and Methods section.