1
Plasmid 26302: pBCN22-R4R3
  • None

  • pCG150
    (Search Vector Database)

  • Worm Expression

  • 7401

  • AttR4

  • Yes

  • AttR3

  • Yes

  • M13 Reverse List of Sequencing Primers

  • M13 (-21) Forward

  • Ampicillin and Chloramphenicol

  • DB3.1

  • 37

  • Use DB3.1 E. coli (must be ccdB resistant). Note that ccdB Survival bacteria (Invitrogen) do not work for this plasmid.

  • Low Copy

  • Puromycin

  • Puromycin resistance gene from pBabePuro (Addgene). rpl-28 promoter and let-858 3'UTR sequences from pPD129.57 vector (Addgene). mCherry gene from pCG144 (Seydoux lab, Addgene). Pharyngeal enhancer (C183) with minimal myo-2 promoter amplified from genomic DNA of AZ218 worms (Caenorhabditis Genetics Center).

  • View sequences (3)
  • View map

  • Ben Lehner

  • UBMTA

Comments: 

Use for puromycin selection of transgenes in C. elegans.
This vector is identical to pBCN21-R4R3, but a 8xC183_minPmyo-2::mCherry::unc-54_3'UTR cassette was inserted in the backbone as an additional visual marker.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Rapid selection of transgenic C. elegans using antibiotic resistance. Semple et al (Nat Methods. 2010 Aug 22. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26302" in your Materials and Methods section.