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Plasmid 26332: pcDNA3.2/V5 mmu-mir-216a
  • mmu-mir-216a

  • mir 216a

  • mir-216a

  • 312

  • M. musculus (mouse)

  • pcDNA3.2/V5-DEST
    (Search Vector Database)

  • Invitrogen

  • Mammalian Expression

  • 7711

  • Gateway Cloning

  • CMV-F List of Sequencing Primers

  • V5 reverse (5′-ACCGAGGAGAGGGTTAGGGAT-3′ )

  • Ampicillin

  • DH5alpha

  • 37

  • Unknown

  • Neomycin

  • View sequences (2)
  • David Bartel

  • UBMTA

Comments: 

pre-miRNA hairpins and the
surrounding regions were amplified from mouse BL6 genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Mammalian microRNAs: experimental evaluation of novel and previously annotated genes. Chiang et al (Genes Dev. 2010 May 15. 24(10):992-1009. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26332" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only