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pcDNA3.2/V5 mmu-mir-216a
(Plasmid #26332)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pcDNA3.2/V5-DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7711
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    mmu-mir-216a
  • Alt name
    mir 216a
  • Alt name
    mir-216a
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    312

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer V5 reverse (5′-ACCGAGGAGAGGGTTAGGGAT-3′ )
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

pre-miRNA hairpins and the
surrounding regions were amplified from mouse BL6 genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.2/V5 mmu-mir-216a was a gift from David Bartel (Addgene plasmid # 26332)
  • For your References section:

    Mammalian microRNAs: experimental evaluation of novel and previously annotated genes. Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP. Genes Dev. 2010 May 15. 24(10):992-1009. 10.1101/gad.1884710 PubMed 20413612