Addgene - pcDNA3.2/V5 mmu-mir-216a Plasmid Data

Plasmid 26332: pcDNA3.2/V5 mmu-mir-216a

Gene/insert name:
mmu-mir-216a
Alt name:
mir 216a
Alt name:
mir-216a
Insert size:
312
Species:
M. musculus (mouse)
Vector backbone:
pcDNA3.2/V5-DEST
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
7711
Cloning method:
Gateway Cloning
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
V5 reverse (5â€²-ACCGAGGAGAGGGTTAGGGAT-3â€² )
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
David Bartel
Terms and Licenses:
MTA

Comments:

pre-miRNA hairpins and the
surrounding regions were amplified from mouse BL6 genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mammalian microRNAs: experimental evaluation of novel and previously annotated genes. Chiang et al (Genes Dev. 2010 May 15. 24(10):992-1009. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26332" in your Materials and Methods section.