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pF151 pcDNA3.1(+)SOD1WT
(Plasmid #26397)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26397 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1 (+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SOD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    470
  • Entrez Gene
    SOD1 (a.k.a. ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    human cDNA insert from cDNA clone cSOD1 from Professor Yoram Groner as described in Lieman-Hurwitzet al., 1982 and Sherman et al 1983.
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

. The complete cDNA insert was subcloned into the vector pET28 and then a PCR amplified the complete cDNA with EcoRI and XhoI sites on the 5’ and 3’ end respectively. This EcoRI-Xho1 0.47kb fragment was cloned into the respective EcoRI and XhoI sites of pcDNA3.1(+).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pF151 pcDNA3.1(+)SOD1WT was a gift from Elizabeth Fisher (Addgene plasmid # 26397 ; http://n2t.net/addgene:26397 ; RRID:Addgene_26397)
  • For your References section:

    Modification of superoxide dismutase 1 (SOD1) properties by a GFP tag--implications for research into amyotrophic lateral sclerosis (ALS). Stevens JC, Chia R, Hendriks WT, Bros-Facer V, van Minnen J, Martin JE, Jackson GS, Greensmith L, Schiavo G, Fisher EM. PLoS One. 2010 . 5(3):e9541. 10.1371/journal.pone.0009541 PubMed 20221404