Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pIBB-mEGFP-IRES-puro2b
(Plasmid #26462)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26462 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIRES-puro2b
  • Backbone size w/o insert (bp) 5829
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pIBB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    196
  • Tag / Fusion Protein
    • mEGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Not1 (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are some discrepancies between the author's sequence and Addgene's quality control sequence, but the depositing lab says that the plasmids should function normally.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIBB-mEGFP-IRES-puro2b was a gift from Daniel Gerlich (Addgene plasmid # 26462 ; http://n2t.net/addgene:26462 ; RRID:Addgene_26462)
  • For your References section:

    CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging. Held M, Schmitz MH, Fischer B, Walter T, Neumann B, Olma MH, Peter M, Ellenberg J, Gerlich DW. Nat Methods. 2010 Sep . 7(9):747-54. 10.1038/nmeth.1486 PubMed 20693996