Addgene - pJC89.3-npy-10 Plasmid Data

Plasmid 26473: pJC89.3-npy-10

Gene/insert name:
npy-10
Insert size:
223
Species:
S. mediterranea (planarian)
GenBank ID:
BK007011
Vector backbone:
pJC53.2
(Search Vector Database)
Vector type:
T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes
Backbone size w/o insert (bp):
3240
Cloning site 5':
Eam1105I
Site destroyed during cloning:
Yes
Cloning site 3':
Eam1105I
Site destroyed during cloning:
Yes
5' sequencing primer:
SP6 List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Amp, Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Phillip Newmark
Terms and Licenses:
MTA

Comments:

dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Genome-wide analyses reveal a role for peptide hormones in planarian germline development. Collins et al (PLoS Biol. 2010 . 8(10):e1000509. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26473" in your Materials and Methods section.