Addgene - pJC54.2-npp-22 Plasmid Data

Plasmid 26493: pJC54.2-npp-22

Gene/insert name:
npp-22
Insert size:
472
Species:
S. mediterranea (planarian)
GenBank ID:
BK007038
Vector backbone:
pJC53.2
(Search Vector Database)
Vector type:
T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes
Backbone size w/o insert (bp):
3240
Cloning site 5':
Eam1105I
Site destroyed during cloning:
Yes
Cloning site 3':
Eam1105I
Site destroyed during cloning:
Yes
5' sequencing primer:
T3 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Amp, Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
EST PL05004B1G05 in pBluescript
Sequence: View sequences (3)
Principal Investigator:
Phillip Newmark
Terms and Licenses:
MTA

Comments:

dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.

The insert has minor sequence discrepancies with NCBI; these do not affect plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Genome-wide analyses reveal a role for peptide hormones in planarian germline development. Collins et al (PLoS Biol. 2010 . 8(10):e1000509. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26493" in your Materials and Methods section.