pLKO.1 MP1 shRNA
(Plasmid #26632)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pLKO.1 puro
  • Backbone manufacturer
    Available at Addgene (#8453)
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    MP1 shRNA
  • Alt name
    MP1
  • Alt name
    MAPKSP1
  • Species
    H. sapiens (human)
  • Entrez Gene
    LAMTOR3 (a.k.a. PRO2783, MAP2K1IP1, MAPBP, MAPKSP1, MP1, PRO0633, Ragulator3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer LKO.1 5'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

shRNA directed against MP1. Target sequence: 5'-GAGATGGAGTACCTGTTATTA-3'

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1 MP1 shRNA was a gift from David Sabatini (Addgene plasmid # 26632)
  • For your References section:

    Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Sancak Y, Bar-Peled L, Zoncu R, Markhard AL, Nada S, Sabatini DM. Cell. 2010 Apr 16. 141(2):290-303. 10.1016/j.cell.2010.02.024 PubMed 20381137