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Plasmid 26632: pLKO.1 MP1 shRNA
  • MP1 shRNA

  • MP1

  • MAPKSP1

  • H. sapiens (human)

  • LAMTOR3 (PRO2783, MAP2K1IP1, MAPBP, MAPKSP1, MP1, PRO0633, Ragulator3)

  • pLKO.1 puro
    (Search Vector Database)

  • Available at Addgene (#8453)

  • Mammalian Expression, Lentiviral, RNAi

  • 7000

  • AgeI

  • Unknown

  • EcoRI

  • Unknown

  • LKO.1 5' List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • Puromycin

  • View sequences (1)
  • David Sabatini

  • UBMTA

Comments: 

shRNA directed against MP1. Target sequence: 5'-GAGATGGAGTACCTGTTATTA-3'

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Sancak et al (Cell. 2010 Apr 16. 141(2):290-303. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26632" in your Materials and Methods section.