pLKO.1 MP1 shRNA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26632||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepLKO.1 puro
Backbone manufacturerAvailable at Addgene (#8453)
- Backbone size w/o insert (bp) 7000
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameMP1 shRNA
SpeciesH. sapiens (human)
Entrez GeneLAMTOR3 (a.k.a. MAP2K1IP1, MAPBP, MAPKSP1, MP1, PRO0633, Ragulator3)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer LKO.1 5' (Common Sequencing Primers)
shRNA directed against MP1. Target sequence: 5'-GAGATGGAGTACCTGTTATTA-3'
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLKO.1 MP1 shRNA was a gift from David Sabatini (Addgene plasmid # 26632)
For your References section:Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Sancak Y, Bar-Peled L, Zoncu R, Markhard AL, Nada S, Sabatini DM. Cell. 2010 Apr 16. 141(2):290-303. 10.1016/j.cell.2010.02.024 PubMed 20381137