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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26688 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCGN
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUL97
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SpeciesHuman Cytomegalovirus (HHV-5)
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MutationKinase Deficient Mutant Lys 355 mutated to Met
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Entrez GeneUL97
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer Bglob-pA-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
UL97 Accession No.: X17403
The UL97 sequence is as it is on PubMed; it was cloned into pCGN with XbaI/KpnI, but there is an addition of the following sites (in order) after the stop codon: EcoRI, EcoRV, HindIII, Bsp106I,/ClaI, HincII/AccI/SalI, XhoI, Apa/EcoO109I, DraII.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HCMV UL97 KD was a gift from Robert Kalejta (Addgene plasmid # 26688 ; http://n2t.net/addgene:26688 ; RRID:Addgene_26688) -
For your References section:
Cyclin-dependent kinase-like function is shared by the beta- and gamma- subset of the conserved herpesvirus protein kinases. Kuny CV, Chinchilla K, Culbertson MR, Kalejta RF. PLoS Pathog. 2010 . 6(9):. 10.1371/journal.ppat.1001092 PubMed 20838604
Map uploaded by the depositor.