Addgene - pLKO.1-puro-cfSec15A

Plasmid 26716: pLKO.1-puro-cfSec15A_5

Gene/insert name:
Dog Sec15A RNAi
Alt name:
EXOC6
Species:
C. familiaris (dog, RNAi)
Mutation:
Dog Sec15A-specific RNAi.
Vector backbone:
pLKO.1-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert (bp):
7000
Cloning site 5':
AgeI
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRI
Site destroyed during cloning:
Yes
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
Keith Mostov
Terms and Licenses:
MTA

Comments:

RNAi only matches dog Sec15A, does not match Sec15B. Does not match other species' Sec15A. RNAi target sequence is: 5'-GCACGGGTCATGATAGTTT-3'.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A molecular network for de novo generation of the apical surface and lumen. Bryant et al (Nat Cell Biol. 2010 Oct 3. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26716" in your Materials and Methods section.