|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26738||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4097
Vector typeMammalian Expression ; reporter for F-actin
Growth in Bacteria
Copy numberHigh Copy
MutationCalponin homology domain of utrophin (aa# 1-261)
/ Fusion Protein
- photoactivatable GFP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The calponin homology domain of utrophin (Utr-CH) was fused to a photoactivatable form of GFP in order to visualize F-actin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PaGFP-UtrCH was a gift from William Bement (Addgene plasmid # 26738 ; http://n2t.net/addgene:26738 ; RRID:Addgene_26738)
For your References section:Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophin. Burkel BM, von Dassow G, Bement WM. Cell Motil Cytoskeleton. 2007 Nov . 64(11):822-32. 10.1002/cm.20226 PubMed 17685442