pNzanI
(Plasmid #26758)

• Depositing Lab: Greg Gloor
• Publication: Bassi et al Biotechnol Prog. 2000 Jul-Aug;16(4):557-63.

Backbone

• Vector backbone: pSD
• Backbone manufacturer: Stan Dunn
• Backbone size w/o insert (bp): 6278
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: inaZ (3'-5' orientation)
• Alt name: ice nucleation protein (INP)
• Species: Pseudomonas syringae
• Insert Size (bp): 4395

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (destroyed during cloning)
• 3′ cloning site: HindIII (destroyed during cloning)
• 5′ sequencing primer: pBRrevBam
• 3′ sequencing primer: pBAD-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: inaZ gene was provided by Dr. Steven Lindow of UC Berkeley from plasmid pUC1813ICE.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

inaZ gene in reverse orientation lacks ice nucleation activity. Use as a control in ice nucleation
tests.

Plasmid contains I445N mutation in the INP sequence. The depositing scientist states that this plasmid behaves as described and has no known functional consequence.