pBF3060
(Plasmid #26853)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pYES2.0
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 7226
  • Vector type
    Yeast Expression, Cre/Lox
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    CreA recombinase
  • Species
    recoded CreA for expression in S. cerevisiae
  • Insert Size (bp)
    1035
  • Mutation
    N/A

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBF3060 was a gift from Nancy DaSilva & Suzanne Sandmeyer (Addgene plasmid # 26853)
  • For your References section:

    A vector set for systematic metabolic engineering in Saccharomyces cerevisiae. Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, Hatfield GW, Da Silva NA, Sandmeyer S. Yeast. 2010 Sep 10. ():. 10.1002/yea.1824 PubMed 20936606