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Addgene

pGMCZ-PrpoB
(Plasmid #27073)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27073 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDE43-MCZ
  • Backbone size w/o insert (bp) 5390
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    zeocin (25 ug/ml)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    P-rpoB
  • Species
    M. smegmatis
  • Insert Size (bp)
    4548

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGMCZ-PrpoB was a gift from Dirk Schnappinger (Addgene plasmid # 27073 ; http://n2t.net/addgene:27073 ; RRID:Addgene_27073)
  • For your References section:

    Protein inactivation in mycobacteria by controlled proteolysis and its application to deplete the beta subunit of RNA polymerase. Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D. Nucleic Acids Res. 2011 Mar;39(6):2210-20. doi: 10.1093/nar/gkq1149 10.1093/nar/gkq1149 PubMed 21075796