Addgene - pCGN-ELK-1 R mut Plasmid Data

Plasmid 27159: pCGN-ELK-1 R mut

Gene/insert name:
ELK1
Species:
H. sapiens (human)
Entrez Gene:
ELK1 ()
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Mutation:
R box mutant: K249A/V250A/E251A
Vector backbone:
pCGN
(Search Vector Database)
Vector type:
Mammalian Expression
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Ron Prywes
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Activation and repression of cellular immediate early genes by serum response factor cofactors. Lee et al (J Biol Chem. 2010 Jul 16. 285(29):22036-49. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27159" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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