pGPS21loxFRTNeo
(Plasmid #27180)

• Depositing Lab: Yoh Wada
• Publication: Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

Backbone

• Vector backbone: pGPS21
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 3000
• Vector type: in vitro transposion
• Selectable markers: Neomycin (select with G418) ; G418 in mouse ES

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Unknown
• Growth instructions: EC100D pir-116 strain
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: lox/Frt/-PGK/EM7pm-G418/Kan-lox/Frt cassette flanked by Tn7L/R
• Species: synthetic
• Insert Size (bp): 2564

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal I (not destroyed)
• 3′ cloning site: Sal I (destroyed during cloning)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid confers Kan resistance (25ug/ml) and Tet resistance (12.5ug/ml). However, it requires pir factor for replication.

Note that this plasmid is supplied on a stab with only Tet. When re-plating, end users should add Kan as well. Also note that the plasmid is only considered high copy in the pir-116 strain. In a WT pir strain, it is considered low copy.