Gene/insert name: Smad3
Insert size: 1300
Species:
H. sapiens (human)
Entrez Gene:
SMAD3
(HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3)
Fusion protein or tag: GST
Terminal: N terminal on insert
Mutation: T179V S204A S208A S213A
Vector backbone:
pGEX-6PSearch Vector Database )
Backbone manufacturer:
Amersham
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4900
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
pGEX-5
List of Sequencing Primers
3' sequencing primer:
pGEX-3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence:
View sequences (1)
Principal Investigator:
Joan Massague
Terms and Licenses:
MTA
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
27222" in your Materials and Methods section.
