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pDestTol2pA2_ubi:EGFP
(Plasmid #27323)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27323 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ubiquitin:EGFP reporter
  • Alt name
    ubi:EGFP
  • Alt name
    ubi-EGFP
  • Alt name
    ubi-GFP
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    4606

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site attB4 (not destroyed)
  • 3′ cloning site attB3 (not destroyed)
  • 5′ sequencing primer attB4
  • 3′ sequencing primer attB3
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

There are a few nucleotide discrepancies between author sequence and Addgene quality control sequence. According to the depositing lab, these do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDestTol2pA2_ubi:EGFP was a gift from Leonard Zon (Addgene plasmid # 27323 ; http://n2t.net/addgene:27323 ; RRID:Addgene_27323)
  • For your References section:

    Ubiquitous transgene expression and Cre-based recombination driven by the ubiquitin promoter in zebrafish. Mosimann C, Kaufman CK, Li P, Pugach EK, Tamplin OJ, Zon LI. Development. 2011 Jan . 138(1):169-77. 10.1242/dev.059345 PubMed 21138979