pGMCK-P38-luxAB-DAS+4
(Plasmid #27376)

• Depositing Lab: Dirk Schnappinger
• Publication: Klotzsche et al Nucleic Acids Res. 2009 Jan 27. ():.

Backbone

• Vector backbone: pDE43-MCK
• Backbone manufacturer: N/A
• Backbone size w/o insert (bp): 4990
• Vector type: Bacterial Expression ; Gateway Destination Vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: MACH 1, 37C
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: luxA, luxBct6
• Insert Size (bp): 2947
• Mutation: N/A

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that Addgene's sequencing results found a single nucleotide insert between bp# 7734/7735, single nucleotide mismatches at bp# 7796 and 7876 and a 16 nucleotide insert between bp# 7877 & 7878 when compared to the full sequence provided by the depositing laboratory. The depositing scientist states that the insertions/mismatches are in regions that are of no functional importance.