pSPL10F13R13::pBIB-BAS (gSPL10)
(Plasmid
#27407)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27407 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBIB-KAN-GW
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Vector typeused to create transgenic lines
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1
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Growth instructionsMACH-1 strain of E. coli (from Invitrogen)
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSPL10
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SpeciesA. thaliana (mustard weed)
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Entrez GeneAT1G27370 (a.k.a. F17L21.15, F17L21_15)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer M13 forward 20 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Created by cloning 4.9-kb (including 1.8 kb upstream of CDS, CDS, and 1.6 kb downstream from CDS) genomic fragment in pENTR/D-TOPO (Invitrogen). This plasmid was recombined with pBIB-KAN-GW using LR clonase (Invitrogen).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSPL10F13R13::pBIB-BAS (gSPL10) was a gift from David Bartel (Addgene plasmid # 27407 ; http://n2t.net/addgene:27407 ; RRID:Addgene_27407) -
For your References section:
MicroRNAs prevent precocious gene expression and enable pattern formation during plant embryogenesis. Nodine MD, Bartel DP. Genes Dev. 2010 Dec 1. 24(23):2678-92. 10.1101/gad.1986710 PubMed 21123653
