|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27665||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5300
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ cloning site SnaB1 (destroyed during cloning)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
untagged (UT) human TERT (hTERT) into pBABEpuro U3-hTR-500 (U3 promoter + hTR sequence given as insert; second insert of hTERT sequence available from GenBank); this plasmid is for coexpression of hTERT and hTR
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBABEpuroUThTERT+U3-hTR-500 was a gift from Kathleen Collins (Addgene plasmid # 27665 ; http://n2t.net/addgene:27665 ; RRID:Addgene_27665)
For your References section:Telomerase RNA level limits telomere maintenance in X-linked dyskeratosis congenita. Wong JM, Collins K. Genes Dev. 2006 Oct 15. 20(20):2848-58. 10.1101/gad.1476206 PubMed 17015423