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pBABEpuroUThTERT+U3-hTR-500
(Plasmid #27665)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27665 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBABEpuro
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UThTERT+U3-hTR-500
  • Alt name
    human telomerase RNA
  • Alt name
    TER
  • Alt name
    TERC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Entrez Gene
    TERC (a.k.a. DKCA1, PFBMFT2, SCARNA19, TR, TRC3, hTR)
  • Entrez Gene
    TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaB1 (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

untagged (UT) human TERT (hTERT) into pBABEpuro U3-hTR-500 (U3 promoter + hTR sequence given as insert; second insert of hTERT sequence available from GenBank); this plasmid is for coexpression of hTERT and hTR

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABEpuroUThTERT+U3-hTR-500 was a gift from Kathleen Collins (Addgene plasmid # 27665 ; http://n2t.net/addgene:27665 ; RRID:Addgene_27665)
  • For your References section:

    Telomerase RNA level limits telomere maintenance in X-linked dyskeratosis congenita. Wong JM, Collins K. Genes Dev. 2006 Oct 15. 20(20):2848-58. 10.1101/gad.1476206 PubMed 17015423