|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27900||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2580
Vector typeEntry vector
Growth in Bacteria
Growth instructionsE. coli Top10 (Invitrogen)
Copy numberLow Copy
Gene/Insert nameWRKY transcription factor encoded by Os03g0741400 LOC_Os03g53050
SpeciesOryza sativa (rice)
Insert Size (bp)1161
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 For
- 3′ sequencing primer M13 Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/D entry vector.
Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUT1235-HQ858818 was a gift from John Gray & Erich Grotewold (Addgene plasmid # 27900 ; http://n2t.net/addgene:27900 ; RRID:Addgene_27900)